Paper Details

In the agricultural sector, a class of chemical compounds known as chlorinated phenol and phenoxyacetic acid is employed as an herbicide, wood preservative, and pesticide. Additionally, they are listed as priority pollutants by the USEPA. The other group of chlorinated phenoxyacetic acid is used as herbicides to control broad leaf weeds in both agriculture and domestic application (to control weeds of home gardens). Following systemic dilution of soil sample on mineral salt medium, three bacterial isolates (D1, D2, and D3), were isolated and subjected to lots of screening of which D2 was proven to be the best. Molecular identification includes polymerase chain reaction, gel electrophoresis, sequence alignment and phylogentic analysis which prove the isolate was Achromobacter sp. Incubation time, substrate concentration, pH, temperature, and inoculum size in mineral salt medium were all the parameters tested during the characterization work 2,4-dichlorophenoxyacetic acid herbicide was used as the alternative source of carbon. The bacterila isolate grew and degrade 2,4-dichlorophenoxyacetic Acid best at 96 h incubation time, 0.72gL-1 substrate concentration, pH of 7.5, 40 ºC temperature, and 400µg/L inoculum size. High performance Liquid Chromatography (HPLC) analysis of the residual 2,4-dichlorophenoxyacetic Acid and the standard solution probe the isolate degrade up to 95.38% of the substrate indicating great potentiality in bioremediation. Keywords: Bioremediation, Achromobater, Herbicide, Agriculture